Twenty Mile Creek is a scenic stream flowing through the Niagara Peninsula of Ontario, Canada, passing through the community of Jordan and draining toward Lake Ontario. The creek winds through one of Ontario's most celebrated wine and agricultural regions, cutting through the Niagara Escarpment — a UNESCO World Biosphere Reserve — before reaching its lower reaches near the lake. The escarpment geography gives the creek a dynamic character, with elevation changes that create varied water types and habitat for a diverse range of fish species. The creek falls under the jurisdiction of the Niagara Peninsula Conservation Authority (NPCA), which manages fishing access and conservation efforts throughout the watershed.