Sixteen Mile Creek is a river in Halton Region within the Greater Toronto Area of Ontario, Canada, flowing from the Niagara Escarpment through the towns of Milton and Oakville before emptying into Lake Ontario. Named for the distance from its mouth to the western end of Lake Ontario, the creek drains a watershed of approximately 372 square kilometres (144 sq mi) and cuts a deep valley through the landscape, creating a diverse riparian corridor home to Carolinian forest habitat. This valley setting, combined with the creek's varied branches and reaches, produces a surprisingly rich fishery that ranges from coldwater trout habitat in the upper branches to productive migratory corridors near Lake Ontario, making it one of the more versatile urban fisheries in the Greater Toronto Area.