Disa Kloof Waterfall is the destination of the Disa Kloof Trail, a short and easy walk within Harold Porter Botanical Garden near Betty's Bay in the Western Cape.

The trail follows the Disa River for approximately 950 metres from the garden entrance to the waterfall — around 30 minutes each way — passing through a wooded, shaded area that shelters a variety of bird species.

The route crosses the Olive May Porter Bridge and is fully accessible for wheelchair users and pushchairs. The trail takes its name from the red disa orchids that bloom on the cliff faces near the waterfall.

A boardwalk with a seating area has been constructed close to the falls, offering a peaceful spot to rest. The Boekenhout Bridge, added after severe flooding in April 2005, carries walkers across a gulley where the original path was washed away.

The trail is particularly popular on hot, windy days when the south-easter blows, as the tree canopy provides welcome shade.