Lotts Creek flows approximately 35 miles through the coastal plain of southeast Georgia, serving as a tributary to the Canoochee River within the Ogeechee River Basin. This blackwater stream winds through Bulloch, Candler, Emanuel, Evans, and Jenkins Counties, creating the iconic southern Georgia landscape of tea-colored water flanked by snow-white sandbars and dense stands of moss-draped cypress and tupelo. The creek flows through low-lying bottomland forests typical of South Georgia's blackwater streams, with regular flooding patterns that influence the surrounding soil and vegetation. The creek forms amenity lakes in some sections and connects to Little Lotts Creek, a tributary that also supports fishing opportunities. Near Statesboro in Bulloch County, the creek passes through mixed habitats including sandhill, flatwoods, bay swamp, and floodplain forests, some of which are protected within the Canoochee Sandhills Wildlife Management Area.