Hollybush Spout is a waterfall on the River Twiss, located along the popular Ingleton Waterfalls Trail in the Yorkshire Dales National Park, England.

It sits between Pecca Falls downstream and the larger Thornton Force upstream, forming part of a sequence of spectacular falls carved through the steep wooded gorge of the Twiss valley.

The water at Hollybush Spout squeezes through rocks before dropping steeply, and reaching it involves climbing steps up from Pecca Falls.

Beyond the spout, the path opens out onto moorland before continuing to Thornton Force, where the River Twiss drops some 14 metres over a limestone cliff.

The full Ingleton Waterfalls Trail, which also takes in falls on the River Doe, runs approximately 8 kilometres and typically takes two and a half to four hours to complete.