Lock 22 Nassau Mills is a boat ramp and lock station on the Trent-Severn Waterway National Historic Site, located at 3779 Nassau Mills Road in Peterborough, Ontario, at kilometer marker 151. 7 (mile 94. 3).

Situated along the scenic River Road drive near Trent University, the site sits alongside the Otonabee River and is referenced on nautical chart #2023.

The surrounding area includes a large expanse of parkland suitable for picnicking, as well as an extensive trail network that connects to the Rotary Greenway Trail and runs both north toward Lakefield and south into Peterborough.

The Lock 22 Nature Area, managed by Trent University, borders the site and offers riverside trails that cross the Otonabee River at the lock.