Upper Colo Reserve is a wilderness campground in the Hawkesbury region of New South Wales, situated beside the Colo River and adjoining the World Heritage–listed Wollemi National Park. The reserve covers 13.

8 hectares, roughly two-thirds of which is native bushland. The Colo Wilderness is recognised as one of approximately 20 remaining true wilderness areas in south-east Australia.

The reserve provides direct access to the Colo River, known for its crystal-clear freshwater and sandy banks. Bookings are essential.

Open fires are prohibited throughout the reserve, and gas BBQs are not permitted; a council-provided electric BBQ is available for cooking.